Indole-3-carboxylic acid, 99%

Substrate for decarboxylase. The formation of indole can be detected by monitoring absorbance. Was used as chromogenic substrate for cloning genes encoding dioxygenases that act on aromatic acids. The p-cumate-degrading strain Pseudomonas putida F1 and the m- and p -toluate-degrading strain P. putida mt-2 transform indole-2-carboxylate and indole-3-carboxylate to colored products identified as indigo, indirubin, and isatin. Dioxygenase is thought to catalyse the first step in this reaction. 1. Eaton R.W., Chapman P.J., Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids. J. Bacteriol. 1995, 177, 6983-8. 2. Yoshida T., Fujita K., Nagasawa T., Novel reversible indole-3-carboxylate decarboxylase catalyzing nonoxidative decarboxylation. Biosci. Biotechnol. Biochem. 2002, 66, 2388-94.