4-MU-NANA*Na

Substrate for the enzymatic assay of neuraminidase. In this assay, neuraminidase catalyses the hydrolysation of 2’-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid to N-acetylneuraminic acid and 4-Methylumbelliferone, a blue fluorescent soluble substance which can be detected fluorometrically. 1. Nayak D.P., Reichl U., Neuraminidase activity assays for monitoring MDCK cell culture derived influenza virus. J. Virol. Methods 2004, 122, 9-15. 2. Potier M., Mameli L., Belisle M., Dallaire L., Melancon S.B., Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate. Anal. Biochem. 1979, 94, 287-96. 3. Wetherall N.T., Trivedi T., Zeller J., Hodges-Savola C., McKimm-Breschkin J.L., Zambon M., Hayden F.G., Evaluation of neuraminidase enzyme assays using different substrates to measure susceptibility of influenza virus clinical isolates to neuraminidase inhibitors: report of the neuraminidase inhibitor susceptibility network. J. Clin. Microbiol. 2003, 41, 742-50. 4. Yen H.L., Hoffmann E., Taylor G., Scholtissek C., Monto A.S., Webster R.G., Govorkova E.A., Importance of neuraminidase active-site residues to the neuraminidase inhibitor resistance of influenza viruses. J. Virol. 2006, 80, 8787-95.