Condensation reagent especially suited for coupling of sterically hindered amino acids. Superior suppression of racemization.

Activation AND Protection SIMULTANEOUSLY
A smart technology for avoiding over-alkylation of primary amines

Guanidino groups play an important role in proteolytic digestion of proteins, as they are essential as initial contact between active pockets of for example trypsin and substrates.

Spermines and other Biogenic Polyamines
interact due to their multi cationic structure with DNA!

• Readily applicable to biolabelling, Click 
  conjugation protocols and peptide synthesis
• Stable under buffer conditions (pH 4-8) 
  and in solid phase peptide synthesis
• Broadly applied in cell und animal 
  imaging, tested in FRET systems
• Available in larger quantities compared 
  to e.g. Alexa, Cy-Dyes etc.
• Purities > 85%, supplied as freeze-dried solid powder

alpha-Sulfo-beta-Alanine has been used to couple to hydrophobic labels like Cyanine and Rhodamine dyes and other hydrophobic residues to increase their solubility in water. As di- or tripeptide a further increase of hydrophilicity can be achieved. Fmoc-beta-Ala(SO3H)-OH can be coupled in SPPS by standard phosphonium or uronium based coupling reagents. In high throughput technologies for DNA sequencing and genomics charge-modified dye-labeled dideoxynucleoside-5’-triphosphates were synthesized for ‘direct-load’ applications in DNA sequencing.

Phacm shows the same stability and orthogonality as Acm and has the additional advantage that it can be deprotected simply by water in the presence of PGA. The addition of co-solvents like acetonitrile helps to improve the solubility of hydrophobic sequences. Water in combination with DMSO (80:20) will make deprotection and cyclization a one pot reaction, which is completed (37°C, pH 7) normally within 16h to 24h.

Lipase B from Candida antarctica (EC 3.1.1.3)
adsorbed on hydrophobic polymer Polystyrene DVB cross linked, 300-700µm,
Synthetic Activity >10.000 U/g (dry), 
very high mechanical stability - suitable for packed bed reactors

Reported applications:

Introduction of 9-fluorenylmethyloxycarbonyl, trichloroethoxycarbonyl, and benzyloxycarbonyl amine protecting groups into O-unprotected hydroxylamino acids using succinimidyl carbonates; Anelka Paquet; Can. J. Chem.; 1982; 60: 976.

Widely Applicable Deprotection Method of 2,2,2-Trichloroethoxycarbonyl (Troc) Group Using Tetrabutylammonium Fluoride; Cheng-yuan Huanga; Ning Wanga; Katsumasa Fujikia; Yuji Otsukaa; Masao Akamatsua; Yukari Fujimotoa; Koichi Fukasea; Journal of Carbohydrate Chemistry 2010; 29(6): 289 – 298.

Cleland's reagent, also known as dithiothreitol or DTT is a water soluble protective reagent for sulfhydryl groups.
It reduces disulfide linkages to free sulfhydryl groups in proteins and enzymes.
It is a component of buffers used in protocols for the isolation and purification of proteins.

In our section RESINS/Mercapto Acids/Thiol Resins:

there are Sulphur carrying building blocks linked through Sulphur to different resin supports. In the case of Amino Thiols the side chains are same as in natural proteinogenic amino acids. The amino group is unprotected and free for any coupling steps via standard SPPS protocols, in order to build up a suitable peptide sequence.
 
After cleavage from the resin (with trifluoroacetate using triethylsilane or ethanedithiol as scavenger) a peptide is being designed, which carries a thiol group on the C-terminus. As sulphur has high affinity to heavy metals, like Zn, Fe or Ni, which are essential metal centers in metallo proteins, the affinity of peptide substrates or pharmacological active sequences can further be increased through a strong sulphur-metal interaction.

H-L-Aha-OH*HCl
H-L-Dab(N3)-OH

4-Azido-L-homoalanine
(S)-2-Amino-4-azidobutanoic acid hydrochloride

Formula:                    C4H8N₄O₂*HCl
Molecular Weight:     144,13*35,45 g/mole

CAS:  942518-29-8

CODE:  HAA5730

1g:       EUR    200,-    US$    300,- 
5g:       EUR    800,-    US$  1200,-

Prices are in EUR, net, exw Germany, shipping not include

Mal-AMCHC-OSu

trans-N-Succinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate

Formula:                    C16H18N₂O₆
Molecular Weight:     334,33 g/mole

CAS:  64987-85-5

CODE:  MAA1000

1g:       EUR    300,-    US$   420,-
5g:       EUR  1200,-    US$ 1680,-

Prices are in EUR, net, exw Germany, shipping not include