Publication

19.12.2022

Incorporation of pseudoproline monomer (Fmoc-Thr[ψMe,Mepro]–OH) facilitates efficient solid-phase synthesis of difficult peptides

Herein we investigate the incorporation of the Fmoc-Thr-pseudoproline monomer during Fmoc SPPS. Typically, pseudoprolines are incorporated as preformed dipeptides. However, the possibility of using the monomer adds flexibility to the synthesis.

Publication

05.09.2022

Solid-Phase Peptide Synthesis Using a Four-Dimensional (Safety-Catch) Protecting Group Scheme

As the molecular diversity of cyclic and branched peptides becomes more and more challenging, a high level of orthogonal dimensionality is required. Herein, we present a new category of orthogonal protecting groups.

Publication

05.09.2022

Solid-Phase Synthesis of an “Inaccessible” hGH-Derived Peptide Using a Pseudoproline Monomer and SIT-Protection for Cysteine

Herein, we report the first-time SPPS of a special peptide sequence via employment of of a serine pseudoproline building block as well as sec-isamyl mercaptan (SIT) as cysteine protecting group.

Publication

27.12.2021

2-Chlorotrityl Chloride and 4-Methylbenzhydryl Bromide Resin Loading Using the Mixture of Organic Solvents: A “Greener” Alternative to Dichloromethane and N,N-Dimethylformamide

Common protocols for the loading of 2-CTC and MBH-Br resins presume the application of DCM, THF, or DMF; these solvents are considered as undesirable in accordance with modern regulatory rules.

Publication

21.12.2021

Easy access to purified complex peptides on larger scales with the peptide easy clean technology – a liraglutide case study

With a remarkable amount of peptide therapeutics already on the market and many more to come, there is an ongoing need to improve manufacturing scale and efficiency for a sustainable, global supply.