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Desthiobiotin-Tyramide
Chemischer Name: N-(4-hydroxyphenethyl)-6-((4R,5S)-5-methyl-2-oxoimidazolidin-4-yl)hexanamide // Synonyme: Desthiobiotin Phenol, (4R,5S)-oxo-N-[2-(4-hydroxyphenyl)ethyl]-5-methyl-2-4-Imidazolidinehexanamide
- Art-Nr.:LS-1660
- CAS Nr.:2242902-55-0
- Formel:C18H27N3O3
- Molare Masse:333,43 g/mol
Startet von 375,00 €
description
Biotin-Tyramide analog that binds less tightly to biotin-binding proteins. It can be displaced competitively by biotin. Streptavidin-based ligands can be gently stripped from desthiobiotin-tyramide-labeled targets with buffered biotin solutions.
references
Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins; T. Cijsouw, A. Ramsey, T. Lam, B. Carbone, T. Blanpied and T. Biederer; 2018; 6: 48. https://www.mdpi.com/2227-7382/6/4/48
Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation; V. Hung, S. S. Lam, N. D. Udeshi, T. Svinkina, G. Guzman, V. K. Mootha, S. A. Carr and A. Y. Ting; Elife D. Pagliarini 2017; 6: e24463. https://doi.org/10.7554/eLife.24463
In Situ Peroxidase Labeling and Mass-Spectrometry Connects Alpha-Synuclein Directly to Endocytic Trafficking and mRNA Metabolism in Neurons; C. Y. Chung, V. Khurana, S. Yi, N. Sahni, K. H. Loh, P. K. Auluck, V. Baru, N. D. Udeshi, Y. Freyzon, S. A. Carr, D. E. Hill, M. Vidal, A. Y. Ting and S. Lindquist; Cell Syst 2017; 4: 242-250 e4. https://doi.org/10.1016/j.cels.2017.01.002
Identification of Microprotein-Protein Interactions via APEX Tagging; Q. Chu, A. Rathore, J. K. Diedrich, C. J. Donaldson, J. R. Yates, 3rd and A. Saghatelian; Biochemistry 2017; 56: 3299-3306. https://doi.org/10.1021/acs.biochem.7b00265
Proximity-dependent labeling methods for proteomic profiling in living cells; C. L. Chen and N. Perrimon; Wiley Interdiscip Rev Dev Biol 2017; 6: e272. https://doi.org/10.1002/wdev.272
Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts; K. H. Loh, P. S. Stawski, A. S. Draycott, N. D. Udeshi, E. K. Lehrman, D. K. Wilton, T. Svinkina, T. J. Deerinck, M. H. Ellisman, B. Stevens, S. A. Carr and A. Y. Ting; Cell 2016; 166: 1295-1307 e21. https://doi.org/10.1016/j.cell.2016.07.041
Directed evolution of APEX2 for electron microscopy and proximity labeling; S. S. Lam, J. D. Martell, K. J. Kamer, T. J. Deerinck, M. H. Ellisman, V. K. Mootha and A. Y. Ting; Nat Methods 2015; 12: 51-4. https://doi.org/10.1038/nmeth.3179
New insights into the DT40 B cell receptor cluster using a proteomic proximity labeling assay; X. W. Li, J. S. Rees, P. Xue, H. Zhang, S. W. Hamaia, B. Sanderson, P. E. Funk, R. W. Farndale, K. S. Lilley, S. Perrett and A. P. Jackson; J Biol Chem 2014; 289: 14434-47. https://doi.org/10.1074/jbc.M113.529578
Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging; H. W. Rhee, P. Zou, N. D. Udeshi, J. D. Martell, V. K. Mootha, S. A. Carr and A. Y. Ting; Science 2013; 339: 1328-1331. https://doi.org/10.1126/science.1230593
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry; M. R. Clutter, G. C. Heffner, P. O. Krutzik, K. L. Sachen and G. P. Nolan; Cytometry A 2010; 77: 1020-31. https://doi.org/10.1002/cyto.a.20970
A. J. Gross and I. W. Sizer; J. Biol. Chem. 1959; 234: 1611.
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