Biotin-PEG(4)-Dde-Alkyne

Chemical name: N-(15-(4,4-dimethyl-2,6-dioxocyclohexylidene)-3,6,9,12-tetraoxa-16-azanonadec-18-ynyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide // Synonyms: Dde-Biotin-Alkyne, Dde-Biotin-PEG(4)-Alkyne, Propargyl-Dde-PEG(4)-Biotin

Art-No.:PEG7980
Cas-no.:1802908-00-4
Formula:C32H50N4O8S
Molecular weight:650,83 g/mol
Gruppiert Produkte - Artikel
Product variant
10 mg
250,00 €
PEG7980.0010
25 mg
415,00 €
PEG7980.0025
100 mg
1.250,00 €
PEG7980.0100
Neu
description
This cleavable trifunctional biotinylating reagent incorporating a cleavable linker is highly valuable for usage in protein enrichment. A linker based on the Dde protecting group leads to efficient release of protein targets under mild aqueous buffered co nditions with 2% hydrazine. The cleaved moiety that remains on the modified peptide only minimally changes the peptide mass and generates an additional positive charge, which facilitates peptide sequencing by ETD.

references
References:

Cleavable trifunctional biotin reagents for protein labelling, capture and release; Yinliang Yang and Steven H. L. Verhelst; Chem. Commun. 2013; 49: 5366-5368. DOI: 10.1039/C3CC42076K.

Comprehensive mapping of O-GlcNAc modification sites using a chemically cleavable tag; Matthew E. Griffin, Elizabeth H. Jensen, Daniel E. Mason, Courtney L. Jenkins, Shannon E. Stone, Eric C. Peters and Linda C. Hsieh-Wilson; Mol. BioSyst. 2016; 12: 1756-1759. DOI: 10.1039/C6MB00138F.

Mapping the Binding Site of BMS-708163 on -Secretase with Cleavable Photoprobes; Gertsik N, Am Ende CW, Geoghegan KF, Nguyen C, Mukherjee P, Mente S, Seneviratne U, Johnson DS, Li YM; Cell Chem Biol. 2017; 24(1): 3-8. DOI: 10.1016/j.chembiol.2016.12.006.

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