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Halo-PEG(4)-Azide
Chemischer Name: 1-Azido-18-chloro-3,6,9,12-tetraoxaoctadecane
- Art-Nr.:RL-3710
- Formel:C14H28ClN3O4
- Molare Masse:337,85 g/mol
Startet von 900,00 €
description
This chloroalkane linker serves as ligand for a specific modified bacterial haloalkane dehalogenase. This is a reporter protein whose sequence can easily be fused to a gene of interest. The reaction between the chloroalkane linker and the enzyme is fast and irreversible under physiological conditions and the system has been used extensively to measure protein-protein interactions in cells.
Furthermore, the additional azide moiety serves clickable moiety.
references
HaloTag technology: a versatile platform for biomedical applications; C. G. England, H. Luo and W. Cai; Bioconjug Chem 2015; 26: 975-86. https://doi.org/10.1021/acs.bioconjchem.5b00191.
HaloTag: a novel protein labeling technology for cell imaging and protein analysis; G. V. Los, L. P. Encell, M. G. McDougall, D. D. Hartzell, N. Karassina, C. Zimprich, M. G. Wood, R. Learish, R. F. Ohana, M. Urh, D. Simpson, J. Mendez, K. Zimmerman, P. Otto, G. Vidugiris, J. Zhu, A. Darzins, D. H. Klaubert, R. F. Bulleit and K. V. Wood; ACS Chem Biol 2008; 3: 373-382. https://doi.org/10.1021/cb800025k.
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