Biotin Tyramide Reagents for Tyrosine-/Protein-Biotinylation

Biotin Tyramide Reagents for Tyrosine-/Protein-Biotinylation

Published on 25/03/2014

Tyramine compounds are converted to highly reactive radicals by horseradish peroxidase in presence of H2O2 which preferentially react with surface exposed tyrosines. Biotin Tyramide and Biotin-PEG-Tyramide therefore are ideal reagents used for tyrosine-/protein-biotinylation.

For long time it is known that tyramine compounds are converted to highly reactive radicals by horseradish peroxidase in presence of H2O2.

In vicinity of proteins, these radicals preferentially react with surface exposed tyrosines. Therefore biotin tyramide is a perfect reagent for protein biotinylation. Numerous applications have been developed and commercialized using this excellent reactivity of biotin tyramide for signal amplification and sensitive detection of very small concentrations.

Biotin tyramide and also a variation with a short PEG spacer in between are available to improve flexibility and solubility.

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