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Chemical name: Glycyl-N-(4-nitrophenyl)-L-prolinamide 4-methylbenzenesulfonate // Synonyms: H-Gly-Pro-pNA*TosOH, Gly-Pro p-nitroanilide p-toluenesulfonate salt, H-Gly-Pro-pNA p-tosylate, (S)-1-(2-aminoacetyl)-N-(4-nitrophenyl)pyrrolidine-2-carboxamide 4-methylbenzenesulfonate
|Molecular weight:||292,29*172,20 g/mol|
Related CAS number: 60189-43-7 (net product)
Used as a chromogenic substrate for dipeptidyl peptidase IV (DPP IV) activity quantification by colorimetric detection of cleaved p-nitroanilide at 405 nm. DPP IV inactivates glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP-1).
Optimization of Poly(N-isopropylacrylamide) as an Artificial Amidase; Yoke-Ming Wong, Yu Hoshino, Kumar Sudesh, Yoshiko Miura, and Keiji Numata; Biomacromolecules 2015; 16(1): 411–421. DOI: 10.1021/bm501671r.
Genetic, enzymatic and metabolite profiling of the Lactobacillus casei group reveals strain biodiversity and potential applications for flavour diversification. E. Stefanovic, K.N. Kilcawley, M.C. Rea, G.F. Fitzgerald, O. McAuliffe; J. Appl. Microbiology 2017; 122(5): 1245-1261. DOI:10.1111/jam.13420.
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