D-Desthiobiotin

Chemical name: (4R,5S)-5-methyl-2-oxo-4-Imidazolidinehexanoic acid // Synonyms: (+)-Dethiobiotin, 5-Methyl-2-oxo-4-imidazolinecaproic acid

  • Product code:LS-1650
  • CAS No.:533-48-2
  • Formula:C10H18N2O3
  • Molecular weight:214,27 g/mol

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description

Biotin analog that binds less tightly to biotin-binding proteins. It can be displaced competitively by biotin. Streptavidin-based ligands can be gently stripped from desthiobiotin-labeled targets with buffered biotin solutions.

references

Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation; J.D. Hirsch et al.; Analytical Biochemistry 2002; 308 (2): 343-357. doi:10.1016/S0003-2697(02)00201-4

A novel tandem affinity purification strategy for the efficient isolation and characterization of native protein complexes; C.J. Gloeckner et al.; Proteomics 2007; 7 (23): 4228-4234. doi:10.1002/pmic.200700038


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