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Chemical name: 4-((2-(2-((6-chlorohexyl)oxy)ethoxy)ethyl)amino)-4-oxobutanoic acid // Synonyms: Haloalkane Dehalogenase Ligand
|Molecular weight :||323,81 g/mol|
Further applications of the HaloTag technology include cellular imaging as well as protein immobilization. Those applications can be accessed via an appropriate modification of the linker's carboxyl terminus, e.g. with an amine-functionalized fluorophore.
Cell Penetration Profiling Using the Chloroalkane Penetration Assay; L. Peraro, K. L. Deprey, M. K. Moser, Z. Zou, H. L. Ball, B. Levine and J. A. Kritzer; J Am Chem Soc 2018; 140: 11360-11369. doi: https://doi.org/10.1021/jacs.8b06144
HaloTag technology: a versatile platform for biomedical applications; C. G. England, H. Luo and W. Cai; Bioconjug Chem 2015; 26: 975-86. doi: https://doi.org/10.1021/acs.bioconjchem.5b00191
HaloTag: a novel protein labeling technology for cell imaging and protein analysis; G. V. Los, L. P. Encell, M. G. McDougall, D. D. Hartzell, N. Karassina, C. Zimprich, M. G. Wood, R. Learish, R. F. Ohana, M. Urh, D. Simpson, J. Mendez, K. Zimmerman, P. Otto, G. Vidugiris, J. Zh u, A. Darzins, D. H. Klaubert, R. F. Bulleit and K. V. Wood; ACS Chem Biol 2008; 3: 373-382. doi: https://doi.org/10.1021/cb800025k
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