description

Trifunctional cross-linker with for conjugation of nucleophiles, like amines, with azides or any other functional groups for click chemistry. This linker bears an additional azirin ring, which will dismiss N2 under illumination of UV light. The remaining carbene will undergo insertion and addition reaction with any neighboring molecules and irreversibly form a covalent bond.

references

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Endeavors to Make the Photophore, Diazirine Easy to Use. Yutaka Sadakane; Yakugaku Zasshi 2007; 127(10): 1693-1699. DOI: org/10.1248/yakushi.127.1693.

Synthesis of ramariolide natural products and discovery of their targets in mycobacteria. Johannes Lehmann, Johannes Richers, Alexander Pöthig, Stephan A. Sieber; Chem. Commun. 2017; 53: 107-110. DOI: 10.1039/C6CC08365J.

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Peptidoglycan Metabolite Photoaffinity Reporters Reveal Direct Binding to Intracellular Pattern Recognition Receptors and Arf GTPases. Wang Y.C., Westcott N.P., Griffin M.E., Hang H.C.;. ACS Chem Biol. 2019; 14(3): 405-414. DOI: 10.1021/acschembio.8b01038.

Cell- and Tissue-Based Proteome Profiling and Dual Imaging of Apoptosis Markers with Probes Derived from Venetoclax and Idasanutlin. Zhu D., Guo H., Chang Y., Ni Y., Li L., Zhang Z.M., Hao P., Xu Y., Ding K., Li Z; Angew Chem Int Ed Engl. 2018; 57(30): 9284-9289. DOI: 10.1002/anie.201802003.

Fishing for Drug Targets: A Focus on Diazirine Photoaffinity Probe Synthesis. James R. Hill, Avril A. B. Robertson; J. Med. Chem. 2018; 61: 6945−6963. DOI: 10.1021/acs.jmedchem.7b01561.