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Spermidine
Chemischer Name: 1,8-Diamino-4-azaoctane // Synonyme: N-(3-Aminopropyl)-1,4-diaminobutane, 99%
- Art-Nr.:LS-1208
- CAS Nr.:124-20-9
- Formel:C7H19N3
- Molare Masse:145,25 g/mol
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description
Spermidine inhibits neuronal nitric oxide synthase (nNOS). It binds and precipitates DNA and may be used for purification of DNA binding proteins. It stimulates T4 polynucleotide kinase and T7 RNA polymerase activity.
Spermidine is a polyamine plant growth regulator that promotes somatic embryogenesis.
references
Blethen S.L., Boeker E.A., Snell E.E., Arginine decarboxylase from Escherichia coli. I. Purification and specificity for substrates and coenzyme. J. Biol. Chem. 1968, 243, 1671-7.
Bouché J.P., The effects of spermidine on endonuclease inhibition by agarose contaminants. Anal. Biochem. 1981, 115, 42-5.
Cull M., McHenry C.S., Preparation of extracts from prokaryotes. Methods Enzymol. 1990, 182, 147-53.
Hu J., Mahmoud M.I., el-Fakahany E.E., Polyamines inhibit nitric oxide synthase in rat cerebellum. Neurosci. Lett. 1994, 175, 41-5.
Karkas J.D., Margulies L., Chargaff E., A DNA polymerase from embryos of Drosophila melanogaster. Purification and properties. J. Biol. Chem. 1975, 250, 8657-63.
Krug M.S., Berger S.L., First-strand cDNA synthesis primed with oligo(dT). Methods Enzymol. 1987, 152, 321-25.
Tabor C.W., Tabor H., Polyamines. Annu. Rev. Biochem. 1984, 53, 749-90.
Wan C.Y., Wilkins T.A., Spermidine facilitates PCR amplification of target DNA. PCR Methods Appl. 1993, 3, 208-10.
Wu W.H., Morris D.R., Biosynthetic arginine decarboxylase from Escherichia coli. Subunit interactions and the role of magnesium ion. J. Biol. Chem. 1973, 248, 1696-9.
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