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Reagents
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Coupling Reagents for Peptide Chemistry
In this news section, we highlight three useful coupling reagents for peptide chemistry: PyCLOCK, PyAOP and HDMA.
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THPTA: Ligand-accelerated Click Chemistry in Aqueous Solution
Polytriazole ligands such as THPTA accelerate the copper-catalyzed azide-alkyne cycloaddition (CuAAC), or “Click”, reaction. Moreover, with the use of further additives, polytriazoles facilitate CuAAC even in live cells by significantly lowering the bioavailability of the copper catalyst.
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Targeted and Reversible Biotinylation with Biotin-SS-Tyramide
Our new Biotin-SS-Tyramide linker is a valuable tool for peroxidase-promoted targeted protein biotinylation. By using peroxidase-tagged antibodies, proteins and protein clusters can be selectively biotinylated, and then isolated using streptavidin. The biotin tag can be subsequently removed using reducing agents (e.g. glutathione).
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Inexpensive & High Quality Guanylation Reagents
We now offer N,N'-Bis-Boc-N"-triflylguanidine, at both a very competitive price and our habitual high quality. Find out about the usefulness of this and our other guanylation reagents!
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Biotin Tyramide Reagents for Tyrosine-/Protein-Biotinylation
Tyramine compounds are converted to highly reactive radicals by horseradish peroxidase in presence of H2O2 which preferentially react with surface exposed tyrosines. Biotin Tyramide and Biotin-PEG-Tyramide therefore are ideal reagents used for tyrosine-/protein-biotinylation.
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Ellmans Reagent
DTNB, frequently called Ellman’s Reagent, used as reagent for determination of free sulfhydryl groups.
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Dess-Martin-Periodinane: Mild Selective Reagent for Oxidation of Alcohols
Dess-Martin-Periodinane is one of the most useful reagents for the reduction of primary and secondary alcohols to aldehydes and ketones.
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Troc-OSu
Reported applications:
Introduction of 9-fluorenylmethyloxycarbonyl, trichloroethoxycarbonyl, and benzyloxycarbonyl amine protecting groups into O-unprotected hydroxylamino acids using succinimidyl carbonates; Anelka Paquet; Can. J. Chem.; 1982; 60: 976.
Widely Applicable Deprotection Method of 2,2,2-Trichloroethoxycarbonyl (Troc) Group Using Tetrabutylammonium Fluoride; Cheng-yuan Huanga; Ning Wanga; Katsumasa Fujikia; Yuji Otsukaa; Masao Akamatsua; Yukari Fujimotoa; Koichi Fukasea; Journal of Carbohydrate Chemistry 2010; 29(6): 289 – 298.
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DTT (Cleland's Reagent)
Cleland's reagent, also known as dithiothreitol or DTT is a water soluble protective reagent for sulfhydryl groups.
It reduces disulfide linkages to free sulfhydryl groups in proteins and enzymes.
It is a component of buffers used in protocols for the isolation and purification of proteins.DTT (racemic), Cleland's Reagentread more
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Hexafluoroacetone as Protecting and Activating Reagent: New Routes to Amino, Hydroxy, and Mercapto Acids and Their Application for Peptide and Glyco- and Depsipeptide Modification
Abstract: A considerable number of biologically active naturally occurring products are peptides, depsipeptides, and peptide conjugates.
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