Fmoc-L-Lys(NDBFOC)-OH

Chemischer Name: N-alpha-(9-Fluorenylmethyloxycarbonyl)-N-epsilon-(1-(3-nitro-dibenzofuran-2-yl)-ethoxycarbonyl)-L-lysine // Synonyme: Fmoc-Lys(NDBFOC)-OH, Fmoc-L-Lys(NDBF)-OH, Fmoc-Lys(NDBF)-OH, 2-(9H-Fluoren-9-ylmethoxycarbonylamino)-6-[1-(3-nitro-dibenzofuran-2-yl)-ethoxycarbonylamino]-hexanoic acid

Art-Nr.:FAA8425
Formel:C36H33N3O9
Molare Masse:651,66 g/mol
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description
Nitrodibenzofuran is a one- and two-photon sensitive protecting group. Masking the amino group of lysine with an NDBF group allows the incorporation of this residue into peptides by standard solid-phase peptide synthesis and selective deprotection by photolysis upon irradiation with UV (365 nm) light or two-photon excitation with near-infrared (800 nm) light. The latter aspect renders this derivative especially useful for biological applications in tissue samples or intact organisms due to increased penetration depth of NIR light as well as lower phototoxicity.

references
Nitrodibenzofuran : A One- and Two-Photon Sensitive Protecting Group That Is Superior to Brominated Hydroxycoumarin for Thiol Caging in Peptides; M. Mohsen Mahmoodi, D. Abate-Pella, T. J. Pundsack, C. C. Palsuledesai, P. C. Goff, D. A. Blank, and M. D. Distefano; J. Am. Chem. Soc. 2016; 138: 5848-5859. https://doi.org/10.1021/jacs.5b11759

The nitrodibenzofuran chromophore: a new caging group for ultra-efficient photolysis in living cells; A. Momotake, N. Lindegger, E. Niggli, R. J. Barsotti, G. C. R. Ellis-Davies; Nat. Methods 2006; 3: 35. https://doi.org/10.1038/nmeth821

Chemical synthesis of a two-photon-activatable chemokine and photon-guided lymphocyte migration in vivo; X. Chen, S. Tang, J.-S. Zheng, R. Zhao, Z.-P. Wang, W. Shao, H.-N. Chang, J.-Y. Cheng, H. Zhao, L. Liu, H. Qi; Nat. Commun. 2015; 6: 7220. https://doi.org/10.1038/ncomms8220

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