MiDye550-Alkyne – ready for Click Conjugation

Properties of the MiDye Chromophore System:

• Readily applicable to biolabelling, Click 
  conjugation protocols and peptide synthesis
• Stable under buffer conditions (pH 4-8) 
  and in solid phase peptide synthesis
• Broadly applied in cell und animal 
  imaging, tested in FRET systems
• Available in larger quantities compared 
  to e.g. Alexa, Cy-Dyes etc.
• Purities > 85%, supplied as freeze-dried solid powder

Spectral and other Properties:

• Absorption 550 nm

• Emission 580 nm

• Extinction coefficient 150.000 l/(mol*cm)

• Quantum Yield 15%

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Find more dyes from UV to NIR in our section Dyes and Fluorescence Labels.

• Biocompatible Functionalized Polyglycerol Microgels with Cell Penetrating Properties; Adam L. Sisson, Dirk Steinhilber, Torsten Rossow, Pia Welker, Kai Licha, and Rainer Haag; Angew. Chem. Int. Ed. 2009; 48: 7540 –7545; DOI: 10.1002/anie.200901583.
• Synthesis, Characterization, and Biological Properties of Cyanine-Labeled Somatostatin Analogues as Receptor-Targeted Fluorescent Probes; Kai Licha, Carsten Hessenius, Andreas Becker, Peter Henklein, Michael Bauer, Stefan Wisniewski, Bertram Wiedenmann, and Wolfhard Semmler; Bioconjugate Chem. 2001;12: 44-50.
• Optical molecular imaging of lymph nodes using a targeted vascular contrast agent; Kai Licha, Niels Debus, Sonja Emig-Vollmer, Birte Hofmann, Michael Hasbach, Dietger Stibenz, Sabine Sydow, Michael Schirner, Bernd Ebert, Diethard Petzelt, Christoph Bührer, Wolfhard Semmler, Rudolf Tauber; Journal of Biomedical Optics 2005; 10(4): 041205.
• A complete substitutional analysis of VIP for better tumor imaging properties; Sarah Bhargava, Kai Licha, Tobias Knaute, Bernd Ebert, Andreas Becker, Carsten Grötzinger, Carsten Hessenius, Bertram Wiedenmann, Jens Schneider-Mergener and Rudolf Volkmer-Engert, J. Mol. Recognit. 2002; 15: 145–153; DOI:10.1002/jmr.565.
• Near-infrared fluorescence imaging with fluorescently labeled albumin: A novel method for non-invasive optical imaging of blood–brain barrier impairment after focal cerebral ischemia in mice; Jan Klohs, Jens Steinbrink, Riad Bourayou, Susanne Mueller, Ryan Cordell, Kai Licha, Michael Schirner, Ulrich Dirnagl, Ute Lindauer, AndreasWunder; Journal of Neuroscience Methods 2009; 180(1): 126-32.
• Receptor-targeted optical imaging of tumors with near-infrared fluorescent ligands; Andreas Becker, Carsten Hessenius, Kai Licha, Bernd Ebert, Uwe Sukowski,Wolfhard Semmler, Bertram Wiedenmann, and Carsten Grötzinger; nature biotechnology 2001; 19: 327-331.