New Dawson Linker Derivatives

New Dawson Linker Derivatives

Published on 11/01/2021

Interested in the formation of peptide thioesters for Native Chemical Ligation? Read on to find out more about the use of Dawson Linker derivatives as thioester surrogates compatible with Fmoc SPPS.

Native chemical ligation (NCL) remains the method of choice for joining two unprotected peptide fragments. In the context of Fmoc solid-phase peptide synthesis (SPPS), the crucial step is the efficient synthesis of C-terminal peptide-thioesters, as the methods established for Boc-SPPS have only limited utility due to the lability of the thioester linkage under basic Fmoc-removal conditions.

The most common strategy for the preparation of a peptide thioester surrogate by Fmoc chemistry is based on the use of a 3,4-diaminobenzoic acid (Dbz) derivative (= Dawson linker). Iris Biotech offers this linker (FAA3165) and various derivatives with a Fmoc protecting group. After Fmoc removal, the resin is being acylated with the first amino acid and peptide elongation can be performed. After successful chain assembly, the resin is activated by reaction of the remaining Dbz amine with para-nitrophenyl chloroformate, followed by rearrangement and cyclization to N-acyl-benzimidazolinone (Nbz) in basic media. Finally, trifluoroacetic acid cleaves the fully deprotected peptide benzimidazolinone which can then be converted to a thioester upon reaction with an aryl thiol.

 

Scheme for the thioester generation by using Me-Dbz during Fmoc SPPS.

The main difficulty during the formation of those thioester surrogates remains the overacylation of the deactivated ortho-amino group of the Dbz linker. Thus, its protection is required to avoid the accumulation of branched and acetylated side products and to better control chain extension.

The methylation of the Fmoc-Dbz amine in position 4 makes it less reactive and suppresses the formation of side products on the para-amino moiety. Thus, MeDbz (FAA3166) allows the rather selective elongation of the peptide chain on the amino function in position 3 compared to the first-generation Dbz linker FAA3165.

Another derivative (FAA3168) is based on the allyloxy-carbonyl (Alloc) protection of the Dbz amine in position 4. After successful synthesis, the Alloc group can quantitatively be removed under mild conditions, and finally the thioester can be formed.

FAA3167 makes use of the tert-butyloxycarbonyl (Boc) group as efficient and acid-labile protecting group for the deactivated Dbz amine. Unlike Alloc, the Boc group can easily be removed by TFA during the global deprotection of the peptide from the resin without the need for an additional step.

Especially for microwave-assisted peptide synthesis, the 4-amino-3-nitrobenzoic acid linker (FAA3169) was developed. After successful chain assembly, Dbz(NO2) can easily be converted into the Dbz linker by on-resin reduction with SnCl2. Even though epimerization of the C-terminal amino acid limits the use of the nitro derivative to the synthesis of peptide-Gly-thioesters, it suppresses the formation of side-products which are otherwise observed during microwave-assisted synthesis when using Dbz or Dbz(Alloc).

 

Dawson linker derivatives provided by Iris Biotech.

Interested in more information on Native Chemical Ligation? -> Please feel free to download our new brochure!

 

References:

  • Synthesis of a New Fluorogenic Substrate for Cystine Aminopeptidase; Y. Kanaoka, T. Takahashi, H. Nakayama, T. Ueno, T. Sekine; Chem. Pharm. Bull. 1982; 30(40): 1485-1487. https://doi.org/10.1248/cpb.30.1485.
  • A Reversible Protection Strategy To Improve Fmoc-SPPS of Peptide Thioesters by the N-Acylurea Approach; S. K. Mahto, C. J. Howard, J. C. Shimko, J. J. Ottesen; ChemBioChem 2011; 12: 2488-2494. https://doi.org/10.1002/cbic.201100472.
  • An efficient Fmoc-SPPS approach for the generation of thioester peptide precursors for use in native chemical ligation; J. B. Blanco-Canosa, P. E. Dawson; Angew. Chem. Int. Ed. Engl. 2008; 47(36): 6851-6855. https://doi.org/10.1002/anie.200705471.
  • Protein Synthesis Assisted by Native Chemical Ligation at Leucine; Z. Harpa, P. Siman, K. S. A. Kumar, A. Brik; ChemBioChem 2010; 11(9): 1232-1235. https://doi.org/10.1002/cbic.201000168.
  • Solid-Phase Synthesis of Head to Side-Chain Tyr-Cyclodepsipeptides Through a Cyclative Cleavage From Fmoc-MeDbz/MeNbz-resins; G. A. Acosta, L. Murray, M. Royo, B. G. de la Torre, F. Albericio; Frontiers in Chemistry 2020; 8: xxx. https://doi.org/10.3389/fchem.2020.00298.
  • Side-Chain Tyr-Cyclodepsipeptides Through a Cyclative Cleavage From Fmoc-MeDbz/MeNbz-resins; G. A. Acosta, L. Murray, M. Royo, B. G. de la Torre, F. Albericio; Front. Chem. 2020; 8: 298. https://doi.org/10.3389/fchem.2020.00298.
  • Chemical protein synthesis using a second generation N-acylurea linker for the preparation of peptide-thioester precursors; J. B. Blanco-Canosa, B. Nardone, F. Albericio, P. E. Dawson; J. Am. Chem. Soc. 2015; 137:  7197-7209. https://doi.org/10.1021/jacs.5b03504.
  • Solid-phase synthesis of homodetic cyclic peptides from Fmoc-MeDbz-resin; S. A. H. A. Monaim, G. A. Acosta, M. Royo, A. El-Faham, B. G. de la Torre, F. Albericio; Tetrahedron Lett. 2018; 59: 1779-1782. https://doi.org/10.1016/j.tetlet.2018.03.084.
  • Benzimidazolinone-Free Peptide o-Aminoanilides for Chemical Protein Synthesis; J. Mannuthodikayil, S. Singh, An. Biswas, A. Kar, W. Tabassum, P. Vydyam, M. K. Bhattacharyya, K. Mandal; Org. Lett. 2019; 21: 9040-9044. https://doi.org/10.1021/acs.orglett.9b03440.
  • Preparation of Peptide o-Aminoanilides Using a Modified Dawson’s Linker for Microwave-Assisted Peptide Synthesis; S. Tsuda, T. Uemura, M. Mochizuki, H. Nishio, T. Yoshiya; Synlett 2017; 28: 1956-1960. https://doi.org/10.1055/s-0036-1588862.
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