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Chemical name: N-(4-aminophenethyl)-biotinamide // Synonyms: Btn-An, Biot-aniline, N-[2-(4-aminophenyl)ethyl]hexahydro-2-oxo-(3aS,4S,6aR)-1H-thieno[3,4-d]imidazole-4-pentanamide, N-(4-aminophenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazol-4-yl]-N-[2-(4- aminophenyl)ethyl]pentanamide
|Molecular weight:||362,49 g/mol|
Biotin-aniline labelling with APEX2 can capture all 13 mitochondrial messenger RNAs while leaving all cyrtoplasmic RNAs untouched. APEX2-mediated Btn-An labeling of RNA is thus a promising method for mapping the subcellular transcriptome, which could shed light on their functions in cell physiology. Hence it provides an alternative methodology to the common Biotin-phenol labelling.
Expanding APEX2 Substrates for Spatial-specific Labeling of Nucleic Acids and Proteins in Living Cells; Ying Zhou, Gang Wang, Pengchong Wang, Zeyao Li, Tieqiang Yue, Jianbin Wang, Peng Zou; Angew. Chem. Int. Ed. 2019; DOI: 10.1002/anie.201905949
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