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Chemical name: N-(4-aminophenethyl)-biotinamide // Synonyms: Btn-An, Biot-aniline, N-[2-(4-aminophenyl)ethyl]hexahydro-2-oxo-(3aS,4S,6aR)-1H-thieno[3,4-d]imidazole-4-pentanamide, N-(4-aminophenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, 5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4- d]imidazol-4-yl]-N-[2-(4- aminophenyl)ethyl]pentanamide
|Molecular weight:||362,49 g/mol|
Biotin-aniline labelling with APEX2 can capture all 13 mitochondrial messenger RNAs while leaving all cyrtoplasmic RNAs untouched. APEX2-mediated Btn-An labeling of RNA is thus a promising method for mapping the subcellular transcriptome, which could shed light on their functions in cell physiology. Hence it provides an alternative methodology to the common Biotin-phenol labelling.
Expanding APEX2 Substrates for Spatial-specific Labeling of Nucleic Acids and Proteins in Living Cells; Y. Zhou, G. Wang, P. Wang, Z. Li, T. Yue, J. Wang, P. Zou; Angew. Chem. Int. Ed. 2019; 58(34): 1763-11767. https://doi.org/10.1002/anie.201905949.
Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics; M. Ke, X. Yuan, A. He, P. Yu, W. Chen, Y. Shi, T. Hunter, P. Zou, R. Tian; Nat. Commun. 2021; 12: 71. https://doi.org/10.1038/s41467-020-20367-x.
Mapping spatial transcriptome with light-activated proximity-dependent RNA labeling; P. Wang, W. Tang, Z. Li, Z. Zou, Y. Zhou, R. Li, T. Xion, J. Wang, P. Zou; Nat. Chem. Biol. 2019; 15(11): 1110-1119. https://doi.org/10.1038/s41589-019-0368-5.
Fabrication and characterization of heparin functionalized membrane-mimetic assemblies; P.-Y. Tseng, S. M. Rele, X.-L. Sun, E. L. Chaikof; Biomaterials 2006; 27(12): 2627-2636. https://doi.org/10.1016/j.biomaterials.2005.10.032.
Quantification of streptavidin adsorption in microtitration wells; L. Välimaa, K. Pettersson, J. Rosenberg, M. Karp, T. Lövgren; Anal. Biochem. 2004; 331(2): 376-384. https://doi.org/10.1016/j.ab.2004.04.003.
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