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Chemical name: Biotin-Ahx-Ahx-Tyramide // Synonyms: Biotin-XX-Phenol, N-(4-hydroxyphenethyl)-6-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanamide
|Molecular weight:||589,8 g/mol|
Reagent for tyramide signal amplification used in many applications including immunohistochemistry, in situ hybridization, electron microscopy, ELISA, and others. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents; improves signal to noise by reducing the titer of a other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications. This reagent is a hydrophobic derivative of the commonly used biotin-tyramide (LS-3500) and can be used to analyze cellular compartments that cannot be biochemically isolated and are challenging to characterize, like the synaptic clefts.
Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging. H.W. Rhee, P. Zou, N.D. Udeshi, J.D. Martell, V.K. Mootha, S.A. Carr, A.Y. Ting; Science 2013; 339: 1328-31. www.sciencemag.org/cgi/content/full/science.1230593/DC1 . DOI: 10.1126/science.1230593.
Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts. Ken H. Loh, Philipp S. Stawski, Austin S. Draycott, Namrata D. Udeshi, Emily K. Lehrman, Daniel K. Wilton, Tanya Svinkina, Thomas J. Deerinck, Mark H. Ellisman, Beth Stevens, Steven A. Carr, Alice Y. Ting; Cell 2016; 166(5): 1295-1307.e21. DOI: org/10.1016/j.cell.2016.07.041
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